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1	Animal Breeding and Reproduction	ABS-7
Quality and Proportion of X and Y Spermatozoa Using Different Sexed Methods in Friesian Holstein Cattle Firlia Safa (a*), Amir Firdaus (a), Putri Utami (b), Aulia Puspita Anugra Yekti (c), Achadiah Rachmawati (c), Trinil Susilawati (c) a) Post Graduate Program Faculty of Animal Science, Brawijaya University, Malang, Indonesia, 65145 *safa.firlia[at]gmail.com b) Doctoral Program Faculty of Animal Science, Brawijaya University c) Lecturer of Faculty of Animal Science, Brawijaya University Abstract Sexing process can damage the spermatozoa membrane, which causes a decrease in the quality of the spermatozoa, so an appropriate method is needed to protect the spermatozoa membrane and maintain its quality. This study aimed to determine the quality of frozen sexed semen using Percoll Density Gradient Centrifugation (PDGC) method from Singosari Artificial Insemination Center and frozen sexed semen using the Albumin Sedimentation (AS) method from Lembang Artificial Insemination Center. Each type of sample was repeated ten times. The variables used were individual motility, viability, abnormality, concentration and total motile spermatozoa (TMS). Data analysis used in this research was a variety of Analysis of Variance, Duncan Multiple Range Test and Chi Square Test. The results of unsexed frozen semen, sexed frozen semen using PDGC method, and sexed frozen semen using AS method showed that individual motility values were (46.0, 41.1, 40.6%), viability (66.1, 59.8, 61.4%), concentration (34.7, 28.5, 12.6 million/straw) and TMS (16.0, 11.7, 5.1 million/straw). The results showed that frozen sexed semen using the PDGC method has qualified the Indonesian National Standard so it can be distributed and feasible to be inseminated. Frozen sexed semen using the AS method on spermatozoa concentration and TMS value has not qualified for the Indonesian National Standard. Keywords: Albumin sedimentation- Friesian holstein- Percoll density gradien centrifugation- Sexed semen- Semen quality- Spermatozoa proportion Share Link | Plain Format | Corresponding Author (Firlia Safa)

2	Animal Breeding and Reproduction	ABS-8
PREGNANCY FAILURE IN FRIESIAN HOLSTEIN COWS USING ALBUMIN SEDIMENTATION METHOD OF FROZEN SEMEN UN-SEXED AND SEXED Tiara Durriyatul Ilmy Siregar (a*), Putri Utami (b), Aulia Puspita Anugra Yekti (c), Nurul Isnaini (c), Sri Wahjuningsih (c),Trinil Susilawati (c) a) Post Graduate Student of Animal Reproduction, Faculty of Animal Science, Brawijaya University, Malang, 65145 * tiarasiregar[at]student.ub.ac.id b) Doctoral student of Animal Reproduction, Faculty of Animal Science, Brawijaya University, Malang, 65145 c)Lecturer of Animal Reproduction, Faculty of Animal Science, Brawijaya University, Malang, 65145 Abstract This research aimed to determine the difference and decrease in the success of artificial insemination using un-sexed frozen semen and sexed frozen semen in the albumin sedimentation method regarding changes in NRR-1 to NRR 2 and NRR 2 to CR. The material 78 Friesian Holstein crossbreed cattle. 39 PFH cattle were inseminated using frozen semen un-sexed,39 inseminated using sexed frozen semen in the albumin sedimentation method.The method study is experimental, collecting primary and secondary data by direct observation in the field. The results the NRR-1 for sexed frozen semen was 95%, NRR-2 87%, resulting in a decrease in sexed semen from NRR-1 to NRR-2 by 8%,un-sexed frozen semen, NRR-1 was obtained by 75% . NRR-2 was 64%,decrease of 11%. In sexed cement, a CR of 62%,resulting in a reduction from NRR-2 to a CR of 25%, while in un-sexed frozen semen, a CR was obtained of 46%, resulting in a decrease from NRR-2 to a CR of 18%. Based on the results of this study, it was concluded that the reduction of the success of artificial insemination using un-sexed frozen semen and sexed frozen semen in the albumin sedimentation method is better from NRR-1 to NRR-2 than NRR-2 to CR. Keywords: Artificial Insemination- Friesian Holstein Filial- Repeat Breeder Share Link | Plain Format | Corresponding Author (Tiara Durriyatul Ilmy Siregar)

3	Animal Breeding and Reproduction	ABS-11
The Effectiveness of Providing Inorganic and Organic Macrominerals on the Macroscopic Quality of Thin-Tailed Sheep Semen Sri Suharyati1*, Muhtarudin3, Kusuma Adhianto1, Siswanto1 and Muhammad Mirandy Pratama Sirat2 1Study Program of Animal Husbandry, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung, Jl. Soemantri Brodjonegoro No. 1, Bandar Lampung, 35145, Indonesia 2Study Program of Animal Nutrition and Feed Technology, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung, Jl. Soemantri Brodjonegoro No. 1, Bandar Lampung, 35145, Indonesia 3Study Program of Doctoral Postgraduate, Faculty of Agriculture, University of Lampung, Jl. Soemantri Brodjonegoro No. 1, Bandar Lampung, 35145, Indonesia *Corresponding author: sri.suharyati[at]fp.unila.ac.id Abstract The objective of this study was to determine the effectiveness of administering inorganic macrominerals (CaCl2 + MgCl2) and organic macrominerals (Ca Lysinate + Mg Lysinate) on the macroscopic quality of Thin-Tailed Sheep semen. The study was conducted at Teaching Farm, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung, using 15 Thin-Tailed Sheep with a Randomized Block Design (RBD) method, employing 3 treatments and 5 replications. Grouping was based on initial body weight. The treatments consisted of P0: 100% Basal Ration- P1: 100% Basal Ration + 1.41 g/kg DM CaCl2 + 0.32 g/kg DM MgCl2- P2: 100% Basal Ration + 25.7 ml/kg DM Ca Lysinate + 6.5 ml/kg DM Mg Lysinate. The observed variables were macroscopic quality, including volume, pH, consistency, and mass motility. Semen volume and pH were analyzed using analysis of variance, while consistency and mass motility were analyzed descriptively. The results indicated that the administration of inorganic and organic minerals did not significantly affect (P>0.05) the volume and pH of Thin-Tailed Sheep semen. The administration of organic minerals (Ca Lysinate and Mg Lysinate) tended to show better volume, consistency, and mass motility. Keywords: Macroscopic quality, Mineral, Semen, Thin-Tailed Sheep Share Link | Plain Format | Corresponding Author (Sri Suharyati)

4	Animal Breeding and Reproduction	ABS-12
The Quality and Proportion of Spermatozoa X and Y in Sexed Semen of Bali Bull with the Percoll Density Gradient Centrifugation Method with Various Gradients using Egg Yolk Tris Aminomethane Diluent Nanda Ayu Rahmawati (a*), Aulia Puspita Anugra Yekti (b), and Trinil Susilawati (b) a) Student of Faculty of Animal Science, Universitas Brawijaya Jalan Veteran No.10-11, Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur 65145 *nandaayur[at]student.ub.ac.id b) Lecturer of Faculty of Animal Science, Universitas Brawijaya Jalan Veteran No.10-11, Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur 65145 Abstract This research aims to determine the quality and proportion of X and Y spermatozoa in sexed semen that separated using the Percoll Density Gradient Centrifugation (PDGC) method with various gradients using egg yolk tris aminomethane diluent. The study was conducted at the Singosari Artificial Insemination Center from December 2023 to February 2024. The material of the study was the semen of 1 bali bull with individual motility &#8805- 70%. The experimental design used a Randomized Group Design with the sexed semen PDGC method treatment: T1 = 10 gradients, T2 = 5 gradients, and T3 = 3 gradients, each with 10 replications. The analysis results showed that the gradient variations had a significant impact on the individual motility of the lower layer of sexed semen (P<0.05). Conversely, gradient variance did not affect of viability, abnormality, concentration, total motile spermatozoa, and individual motility of the upper layer of sexed semen (P>0.05). Regarding the proportion of sexed X and Y spermatozoa, the results were as follows: T1 = 85.40% and 79.80%- T2 = 81.20% and 77.90%- T3 = 75.10% and 79.90%. In conclusion, sexed semen using the PDGC method with varied gradients is suitable for the freezing process due to its high quality. Keywords: Bali Bull, Percoll Density Gradient Centrifugation, Sexed Semen, Tris Aminomethane + 20% Egg Yolk Diluent Share Link | Plain Format | Corresponding Author (Nanda Ayu Rahmawati)

5	Animal Breeding and Reproduction	ABS-17
Reproduction performance of imported dairy cows and its first offspring in BBPTU-HPT Baturraden, Purwokerto Henny Leondro1,*, Heri Supriyadi2, Yuli Arif Tribudi3 1 Faculty of Animal Science, Universitas PGRI Kanjuruhan Malang, Malang 65148, Indonesia 2 BBPTU-HPT Baturraden (National Breeding Center and Forages Baturraden), Purwokerto, Indonesia 3 Faculty of Agriculture, Universitas Tanjungpura, Pontianak Abstract The purpose of this study was to compare the reproduction performance of imported dairy cows (G0) and their first offspring (G1) in the first lactation. This study used secondary data collected by BBPTU-HPT Baturraden, Purwokerto, from April to August 2020. The variables observed were age at first partus, service per conception (S/C), days open and calving interval. The data was analyzed using an unpair test (independent sample t-test) to compare the mean between G0 and G1. The results showed that there are no significant differences in reproduction performances in G0 and G1. However, the G1 cows had the higher values in all parameters. The mean values of age at first partus, S/C, days open, and calving interval of G0 and G1 cows were 772.48 and 793.31 days, 2.20 and 2.39 times, 209.94 and 245.91 days, 488.21 and 494 days, respectively. It could be concluded that the reproduction performance of G0 and G1 are relatively similar. Keywords: Reproduction, performance, dairy cows, imported, first generation Share Link | Plain Format | Corresponding Author (Henny Leondro)

6	Animal Breeding and Reproduction	ABS-25
Quality and Proportion of Bali Bull Spermatozoa Using The Percoll Density Gradient Centrifugation Method on Different Gradients with Andromed Diluent Aditiya Wahyudi(a*), Aulia Puspita Anugra Yekti(b*), and Trinil Susilawati(b*) a) Student at Fakultas Peternakan Universitas Brawijaya jalan Veteran No.10-11, Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur 65145 b) Lecturer at Fakultas Peternakan Universitas Brawijaya jalan Veteran No.10-11, Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur 65145 Email: aditiyawahyudi[at]student.ub.ac.id, auliapay[at]ub.ac.id, and tsusilawati[at]ub.ac.id Abstract This study aims to determine the quality and proportion of Bali Bull semen used the Percoll density gradient centrifugation (PDGC) method at different gradients with Andromed diluent. The study was conducted at Singosari Artificial Insemination Center from December 2023 to February 2024. The experimental method used was a Randomized Group Design with sexed semen PDGC method treatment. The treatment in the study was T1: 10 Gradient, T2: 5 Gradient, and T3: 3 Gradient with 10 replications. Statistical analysis showed highly significant differences (P<0.01) in differences gradient treatments in the lower layer of individual motility. The results showed that the percentage of motility resulting from sexing in the lower layer obtained the highest value in T1 77, 86 %,while in the upper layer the highest percentage of motility was obtained in T3 which is 61,72% Meanwhile, in terms of viability, abnormalities, total motile spermatozoa and concentration, there were no differences (P>0.05) in differences gradient treatments. While the proportion of spermatozoa in sexed X and Y, T1=77.6% and 84,8%- T2=75% and 81,8%- T3=75,8% and 79,1%. In conclusion, the quality of sexed semen is categorized as good, in terms of the parameters of motility, concentration and total motile spermatozoa. Keywords: Andromed, bali cattle, percoll density gradien centrifugation, sexing, quality. Share Link | Plain Format | Corresponding Author (Aditiya Wahyudi)

7	Animal Breeding and Reproduction	ABS-29
Characterization and Correlation of Udder Morphology with Milk Production of Etawah Crossbreed (PE) Goat. Ahmad Fahrudin Husen1, Suyadi2, Veronica Margareta Ani Nurgiartiningsih2* 1Graduate Program, Faculty of Animal Science, Brawijaya University, Malang 65145, East Java, Indonesia. 2Faculty of Animal Science, Brawijaya University, Malang 65145, East Java, Indonesia *Corresponding author: vm_ani[at]ub.ac.id Abstract The Etawah Crossbred Goat (PE) demonstrated potential as a commercial dairy goat in tropical regions susceptible to heat stress. However, information regarding milk production and udder-teat morphology in PE goats was limited. This study aims to characterize udder-teat morphology and evaluate its relationship with milk production. Data were collected from 87 lactating female PE goats at BPTU-HPT Pelaihari, South Kalimantan. Nine variables were measured: daily milk yield (DMY), teat length before and after milking (TLB, TLA), udder length before and after milking (ULB, ULA), udder width before and after milking (UWB, UWA), and udder circumference before and after milking (UCB, UCA). The study employed t-tests, single-factor ANOVA followed by Duncans test for significant results, and Pearson correlations for statistical analysis. Results revealed that lactation age was highly significantly affected (P<0.01) on DMY. Goats <90 days post-weaning produced an average of 0.52 liters, while those >90 days post-weaning yielded 0.36 liters. Notably, the study found highly significant positive correlations (P<0.01) between DMY and UWB (r=0.589), UCB (r=0.565), UWA (r=0.401), and UCA (r=0.273). In conclusion, this research contributes valuable insights for improving dairy goat farming in tropical regions. Characterizing udder-teat morphology and its relationship with daily milk production can serve as a foundation for targeted breeding programs to enhance milk productivity in PE goats. Keywords: Characterization, Etawah Crossbred Goat, Udder-Teat Production, Milk Production Share Link | Plain Format | Corresponding Author (Ahmad Fahrudin Husen)

8	Animal Breeding and Reproduction	ABS-40
Polymorphisms Analysis Of BMP15 and GDF9 Gene in Dorper Sheep By Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Technique Asrullah As1, Tri Eko Susilorini1, Wike Andre Septian1, Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro1, Ani Atul Arif1, and Suyadi1* 1Faculty of Animal Science, Brawijaya University, Malang 65145, Indonesia Abstract This study examined BMP15 and GDF9 gene polymorphisms in Dorper sheep and their potential link to prolificacy traits. Blood samples from 17 Dorper ewes with recorded litter sizes were analyzed using PCR-RFLP with HAEIII and HhaI restriction enzymes for BMP15 and GDF9 genes, respectively. Despite observed litter size variations (70.59% single births, 29.41% twin births), PCR-RFLP analysis revealed monomorphic patterns for both genes across all samples. All individuals exhibited wild-type genotypes, with no genetic variation detected at the examined restriction sites. Genotype and allele frequencies were 1 for both genes, resulting in zero heterozygosity and polymorphic information content (PIC) values. These findings suggest that the PCR-RFLP method with the selected restriction enzymes may not effectively detect prolificacy-related genetic variations in Dorper sheep. The discrepancy between genetic homogeneity and phenotypic variability in litter size indicates that other genes or molecular mechanisms may influence prolificacy traits in this breed. Further research using larger sample sizes, alternative genetic markers, and more sensitive techniques is recommended to elucidate the genetic basis of prolificacy in Dorper sheep. Keywords: Dorper Sheep, BMP15 Gene, GDF9 Gene, Litter Size, PCR-RFLP Share Link | Plain Format | Corresponding Author (Asrullah As)

9	Animal Breeding and Reproduction	ABS-54
Preliminary SNP detection of HSPA1A gene in Indonesian local sheep Aprilianna Putri Zahara Nafsina Luvita Sari1 and Alek Ibrahim2 1 Faculty of Animal Husbandry, Padjadjaran University, Sumedang, Indonesia 2 Research Center of Animal Husbandry, Organization of Food and Agriculture, National Research and Innovation Agency (BRIN) Abstract Heat shock proteins (HSPs) are a collection of proteins that promote thermotolerance in cells and protect against environmental stress. This study was a preliminary analysis to identify the nucleotide variant within the partial HSPA1A gene in several Indonesian sheep breeds. The blood samples were collected from five breeds of Indonesian sheep (Sapudi, Batur, Sakub, Wonosobo, and Garut) to be analyzed and amplified using a specific primer. As a result, based on the sequence alignment and direct observation of the electropherograms, three variants were found, namely SNP c.268A>G, c.503A>G, and c.733A>G. All SNPs were categorized as synonymous mutations since they did not alter the amino acid. The genotyping of samples showed two types of genotypes from each SNP. In conclusion, our study suggests exploring other regions of the HSPA1A gene to find candidate mutations for further genetic diversity studies. Keywords: Heat shock protein, HSPA1A, sheep, Indonesia, SNP Share Link | Plain Format | Corresponding Author (Aprilianna Putri Zahara Nafsina Luvita Sari)

10	Animal Breeding and Reproduction	ABS-60
Phenotypic characterization of six different Indonesian local chicken breeds Yuli Arif Tribudi (a,b), Alek Ibrahim (c), Aprilianna Putri Zahara Nafsina Luvita Sari (d), Irwan Lovadi (e), Richard Crooijmans (f), Veronica Margareta Ani Nurgiartiningsih (b*) a) Department of Animal Science, Universitas Tanjungpura, Pontianak, Indonesia b) Department of Animal Science, Universitas Brawijaya, Malang, Indonesia *vm_ani[at]ub.ac.id c) Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Bogor, Indonesia d) Faculty of Animal Husbandry, Universitas Padjadjaran, Sumedang, Indonesia e) Department of Biology, Faculty of Mathematics and Natural Science, Universitas Tanjungpura, Pontianak, Indonesia f) Department of Animal Breeding and Genomic, Wageningen University and Research, The Netherlands Abstract Identifying and studying local chicken genetic resources in Indonesia is critical for conservation, and improving chicken breeding initiatives. The objective of this study was to determine the morphometric characteristics of Tukong chicken offspring in West Kalimantan related to finding the genetic variation and its relationship among chickens that was spreading in Indonesia. This study used 203 chickens (73 males and 130 females) of six chicken breeds: Nunukan, Tukong, and Kampung from Kalimantan, Merawang from Bangka Belitung, and two populations from Java: Tukong_like and Kampung_like. All chickens were included in the observation and measurement. The morphological data were tabulated and analyzed with descriptive statistics. The morphometric data were used for one-way analysis of variance to test the effect of breeds on body measurement in chickens. In total populations, the majority of chickens have red comb color (95%), colored feathers (92%), and read earlobe color (87%) whereas around 50% of the chicken did have a yellow shank color (49%), yellow beak color (50%), orange eye color (42%) and a single comb type (50%). The population effect on BW and morphometric traits significantly differed (p<0.05) in both male and female chickens. In both sex groups, the Tukong had the lowest BW (male = 1.59+-0.23 kg, female = 1.29+-0.21 kg), whereas the Kampung from Java had the highest BW (male = 2.66+-0,44 kg, female = 2.08+-0.30 kg). All female groups are smaller than the male groups in all six chicken populations, except for BcL (in Nunukan chicken) and BeL (in Merawang chicken). The male Tukong had the lowest results in all measurements except in BrL. Meanwhile, female Tukong only had the lowest point of the five traits (BrC, BcL, WL, BeL, and BW). Tukong chickens have lower morphometric measurements than the other five local chickens. There is a mixed value between Kampung from Java chickens and Tukong from Java chickens, indicating that there is interbreeding betwe Keywords: Indonesian local chicken, Tukong chicken, morphometric, phenotypic Share Link | Plain Format | Corresponding Author (Alek Ibrahim)

11	Animal Breeding and Reproduction	ABS-62
Effects of fermented coconut dregs and rumen contents mixture as rearing substrates on prepupae and pupae development of black soldier fly Nurul Isnaini1,*, Akbar Aditya Trihendarsa1, and Faizal Andri1 1Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *Corresponding author: nurulisna[at]ub.ac.id Abstract Black soldier fly (Hermetia illucens) are increasingly utilized in sustainable waste management and as a high-protein feed source in the livestock industries. Optimizing their growth conditions, particularly using various organic substrates, is crucial for enhancing their development and maximizing biomass yield. This study aimed to evaluate the effects of fermented coconut dregs (FCD) and rumen contents (FRC) mixture as rearing substrates on prepupae and pupae development of black soldier fly. Method used in this study was an experiment with five treatments and five replicates. The treatments were various proportions of FCD and FRC mixtures including T0: 100% FCD, T1: 95% FCD + 5% FRC, T2: 90% FCD + 10% FRC, T3: 85% FCD + 15% FRC, and T4: 80% FCD + 20% FRC. Results showed that various proportions of FCD and FRC mixtures significantly affect (P < 0.05) prepupae development. Increasing the proportion of FRC reduced (P < 0.05) the length, width, and weight of prepupae, with T4 showing the lowest value. Pupae development also significantly differed (P < 0.05) among rearing substrates. Increasing the proportion of FRC leads to a reduction in pupae length, width, and weight, with T4 resulted in the lowest pupae development traits. These findings highlight that while FRC can be incorporated into the rearing substrate, higher proportions may hinder optimal prepupae and pupae development of black soldier fly. Keywords: black soldier fly, organic substrate, prepupae development, pupae development sustainable waste management Share Link | Plain Format | Corresponding Author (Nurul Isnaini)

12	Animal Breeding and Reproduction	ABS-67
Growth performances of black soldier fly larvae reared on the different ratio of fermented waste Akbar Aditya Trihendarsa1, Faizal Andri1, and Nurul Isnaini1,* 1Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *Corresponding author: nurulisna[at]ub.ac.id Abstract Black soldier fly (BSF) is increasingly recognized as a vital component of circular economy systems, facilitating organic waste recycling while producing alternative protein feed for the livestock industry. This study aimed to evaluate the growth performances of BSF larvae reared on varying ratios of fermented waste consisted of fermented coconut dregs (FCD) and fermented rumen contents (FRC). Five treatments were tested, namely T0 (100% FCD), T1 (95% FCD + 5% FRC), T2 (90% FCD + 10% FRC), T3 (85% FCD + 15% FRC), and T4 (80% FCD + 20% FRC). Larval length, width, and mass were measured at 6, 11, and 16 days. Data were analyzed using one-way analysis of variance, with significance set at P < 0.05. Duncan Multiple Range Test was used to differentiate treatment effects. Results indicated that larvae reared on 100% FCD (T0) consistently exhibited the highest values for length, width, and mass across all time points (6, 11, and 16 days). A significant decline (P < 0.05) in growth parameters was observed as the proportion of FRC increased, with the lowest performance recorded in the T4 treatment (80% FCD + 20% FRC). In conclusion, 100% FCD is recommended as the optimal substrate for maximizing BSF larval growth. Keywords: black soldier fly, coconut dregs, larvae development, rumen contents, sustainable waste management Share Link | Plain Format | Corresponding Author (Nurul Isnaini)

13	Animal Breeding and Reproduction	ABS-70
Identification Of IGF1 Gene Polymorphisms Using PCR-RFLP In Indigenous Goats Of East Java Wike Andre Septian1 , Ari Ardiantoro1, Kuswati1, Muhaimin Rifa^i2, Ale1, and Suyadi1 1Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia 2Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang 65145, Indonesia Abstract Abstract. The objective of this study was to identify insulin growth factor (IGF-1) gene polymorphism using PCR RFLP technique in order to improve growth evaluation. The insulin-like growth factor 1 (IGF1) gene is a critical factor in the growth and development of goats. It influences a range of traits, including body size, milk production, and reproductive performance. This gene was therefore identified as a potential candidate gene. Blood samples were collected from 120 local goats of East Java, namely 40 etawa goats, 40 senduro goats, and 40 peanut goats. The DNA was extracted using the Geneaid DNA Purification Kit. The IGF-1 polymorphisms were determined by the PCR-RFLP method, employing the HaeIII restriction enzyme. The primers for the 398 bp long IGF1 gene are forward 3^ TTGAACAGACAAGCCCAC and reverse 5^ CTCCCTTCTACTGCTGTG. The results demonstrated that the local goat population in East Java is in Hardy-Weinberg equilibrium. The conclusion of this study is that the IGF-1 gene in local goats in East Java is polymorphic, and this diversity information can be employed for association studies with growth traits, litter size, and twinning rates. Keywords: IGF1 gene- Indigenous Goats- PCR-RFLP Share Link | Plain Format | Corresponding Author (wike andre septian)

14	Animal Breeding and Reproduction	ABS-78
Morphometric Comparison of Body Weight and Shank Length in Day-Old Mojosari and Alabio Ducks Ardian Fitra (a*), Gatot Ciptadi (b), V. M. Ani Nurgiartiningsih (b) a) Student of Animal Breeding and Reproduction, Faculty of Animal Science, University of Brawijaya, Jalan Veteran 10-11, Malang 65145, Indonesia *ardianfitra15[at]student.ub.ac.id b) Departement of Animal Breeding and Reproduction, Faculty of Animal Science, University of Brawijaya, Malang 65145, Indonesia Abstract This study focused on the morphometric comparison of body weight and shank length in day-old Mojosari and Alabio ducks. Analysis revealed minor differences in body weight and shank length between males and females within both breeds, with Mojosari ducks exhibiting higher variability. While body weight did not significantly differ between breeds, female Mojosari ducks had longer shanks compared to Alabio females. A moderate positive relationship between body weight and shank length was observed in both breeds. These findings contribute to understanding the morphometric traits of Mojosari and Alabio ducks, with implications for breed improvement and selection in duck production systems. Keywords: Alabio Ducks-Mojosari Ducks-Morphometric Share Link | Plain Format | Corresponding Author (Ardian Fitra Fitra)

15	Animal Breeding and Reproduction	ABS-86
PRODUCTION PERFORMANCE OF KACANG GOATS IN TUBAN, INDONESIA Kuswati, Rizki Prafitri, Doni Herviyanto and Tri Eko Susilorini Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract Goat performance is considered important for the success of the livestock business. Kid Crop and Natural Increase are indicators of an area^s livestock production efficiency. The study aimed to examine the production of Kacang goats doe based on population structure, Kid crop, and Natural Increase. This research was carried out in the Milwan area, Tambakrejo District, Tuban Regency, which is the source of Kacang goat livestock. This study aimed to examine goat performance and production based on population structure, Kid Crop, and Natural Increase. The research materials included 177 Kacang goats, divided according to the physiological status of bucks, does, yearlings, and kids. This research method was a case study in the field with a qualitative and quantitative approach. Location selection was done using purposive sampling and the snowball sampling method. The variables observed were broodstock production based on qualitative characteristics, population structure, Kid Crop, and Natural Increase. Descriptive data analysis was used. The results of the study showed that the qualitative characteristics of Kacang goats were dominated by mixed colors (33%), black (28%), and brown (20%). The face profile is straight, the direction of the ears is upright semi-hanging, horned with a straight horn direction, and the udder shape of the jug. The population structure was dominated by adult females (36.75%) and males (2.56%). Based on gender, the herd population structure of children and young adults is 17.95%, 7.69%, and 2.56%, respectively, and females are 30.77%, 4.27%, and 36.75%, respectively. Kid Crop by 164% and Natural Increase by 48.72%. It was concluded that the population structure was dominated by adult females with Kid Crop and Natural Increase in the high range. Keywords: Kacang goats, Kid Crop and Natural Increase Share Link | Plain Format | Corresponding Author (Rizki Prafitri)

16	Animal Products and Sustainable Post-Harvest Technology	ABS-1
The effect of adding black tea essential in soaking salted egg on water content, salt content, water activity (Aw) and yolk color Dedes Amertaningtyas, Anif Mukaromah Wati, Eka Nurwahyuni, Rismarini Hapsari, and Rischa Amalia Saleha Animal Science Faculty, Universitas Brawijaya, Malang 65145, Indonesia Abstract The research objective to determine the effect of adding black tea (Camellia sinensis) essence using the wet method. The research method was a laboratory experiment and counting used Completely Randomized Design (CRD) of 4 treatments and 4 replications. Data were analyzed using analysis of variance (ANOVA) and if differences were found, continued with Duncan^s Multiple Range Test (UJBD). The treatments given were T1 (without adding black tea), T2 (adding 25% black tea), T3 (adding 50% black tea) and T4 (adding 75% black tea). Variables included water content (%), salt content (%), water activity (Aw), and salted egg yolk color. The results showed that the addition of black tea leaf extract did not have a significant effect (p>0.05) on water content, salt content, Aw, and color of salted egg yolk. The conclusion of this research is that the addition of black tea essence in making salted chicken eggs using the wet method has no effect on the values of water content (61.39-66.31%), salt content (1.08-1.51%), Aw (0.91-0.97), and yellow color (L* 75.30-76.85, a* 3.88-5.23 and b* 41.75-43.65) of salted eggs. Keywords: Black tea, egg, salted egg, tannin, wet method Share Link | Plain Format | Corresponding Author (Dedes Amertaningtyas)

17	Animal Products and Sustainable Post-Harvest Technology	ABS-2
The quality of marshmallow candy based on beef bone gelatin with the addition of powdered cream milk on texture, water content, Aw and pH Dedes Amertaningtyas1*, Faiza Bunga Risti2, Eny Sri Widyastuti1, Herly Evanuarini1, Khothibul Umam Al Awwaly1, Dewi Masyithoh3 and Rischa Amalia Saleha4 1Lecturer, Animal Science Faculty, Universitas Brawijaya, Malang 65145, Indonesia 2Student, Animal Science Faculty, Universitas Brawijaya, Malang 65145, Indonesia 3PT. Kembang Joyo Sriwijaya, Karang Ploso, Malang 65152, Indonesia and Animal Husbandry Faculty, Islamic University of Malang, Malang 65144, Indonesia 4Postgraduate, Animal Science Faculty, Universitas Brawijaya, Malang 65145, Indonesia Abstract The addition of powdered milk cream to beef gelatin marshmallows containing fat is used to improve the quality of the marshmallows. The research objective was to determine the effect of adding powdered cream milk. The material used in this research was beef gelatin. Marshmallow added with powdered cream milk at levels of 0%- 25% and 50%. The research method used was experimental research and counting used completely randomized design using 3 treatments and 4 replications. The variables were texture, water content, Aw and pH. The results showed that the average texture at each level was 2.325 N- 2.275 N- 4.125 N- water content: 21.50%- 22.25%- 23.75%- Aw: 0.811- 0.800- 0.791- and pH 6.55- 6.5- 6.28. The conclusion from this research known that the quality of marshmallow candy with the addition of 25% powdered cream milk produces the best candy quality in terms of texture, water content, Aw and pH by producing values of 2.275 N, 22.25%, 0.80 and 6.5 respectively. Keywords: Full cream, gelatin, marshmallow, soft candy, quality Share Link | Plain Format | Corresponding Author (Dedes Amertaningtyas)

18	Animal Products and Sustainable Post-Harvest Technology	ABS-9
Enhancing the quality of beef sausage by using papaya peel flour as a filler Herly Evanuarini*, Agus Susilo, Premy Puspitawati Rahayu Department of Livestock Product Technology, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *herlyfptub[at]ub.ac.id Abstract The focus of this research is the development of papaya peel flour as a filler in beef sausages. Using papaya peel flour as an additional ingredient in beef sausages can increase the added value of agro-industrial waste. This research aimed was to evaluate the quality of beef sausages with the addition of papaya peel flour. The research material is beef sausage made from beef with papaya peel flour and other optional ingredients. The research method used was an experiment in the laboratory using a completely randomized design with 4 treatments and repeated 5 times. Beef sausages incorporated with papaya peel flour were formulated with the addition of 2%, 4%, 6% (of the total meat used), and without addition as a control. Beef sausage quality parameters were evaluated from pH, cooking loss, water holding capacity, yield, color L (lightness), a* (redness), and b* (blueness). The experimental results showed that papaya peel flour had a very significant effect (p<0.01) on the quality of beef sausage. Papaya peel flour can increase pH value, water holding capacity, yield, redness (a*), and blueness (b*) of beef sausage. A decrease occurred in the cooking loss, and brightness (L). Based on the research results obtained, incorporating beef sausage with 6% papaya peel flour produces the best quality. Keywords: Beef sausage- Papaya peel flour- Filler Share Link | Plain Format | Corresponding Author (Herly Evanuarini)

19	Animal Products and Sustainable Post-Harvest Technology	ABS-10
Total plate count analysis of Gouda cheese with different drying method and drying time Nuning Nur Laila, Abdul Manab, Hari Dwi Utami, Lilik Eka Radiati Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract Drying is an effective method to increase the shelf life of Gouda cheese due to its high moisture and protein contents, which are ideal for the growth of microorganisms. This study aimed to measure the microbial content of Gouda cheese dried using different drying methods and drying time. The 2x2 factorial design was used: drying method (oven and dry roasting) and drying time (60 and 120 minutes). The observed variable was the Total Plate Count (TPC). As a result, only the different drying methods showed a significant influence (p<0.01) on the TPC of dried Gouda cheese. The dry roasting method had a lower TPC (1.94 Log CFU/g) than the oven method (2.57 Log CFU/g). Meanwhile, the lowest value of TPC (1.58 Log CFU/g) was reached by the dried Gouda cheese with a dry roasting method in 60 minutes drying time. In conclusion, the dry roasting method could be recommended for producing dried Gouda cheese. Keywords: Gouda cheese, drying, Total Plate Count, dry roasting Share Link | Plain Format | Corresponding Author (Nuning Nur Laila)

20	Animal Products and Sustainable Post-Harvest Technology	ABS-21
Utilization of Local by-products for Functional Dairy Products Aju Tjatur Nugroho Krisnaningsih1*, Premy Puspitawati Rahayu2, Maris Kurniawati1 1.Faculty of Animal Husbandry, Universitas PGRI Kanjuruhan Malang, Jl. S. Supriadi 48 Malang, 65148, Indonesia. 2.Animal Product Technology, Faculty of Animal Science, Universitas Brawijaya, Malang, 65141, Indonesia *Corresponding Author: email: ajutjatur[at]unikama.ac.id Abstract Changes in people^s lifestyles through the implementation of a good food diet are carried out by consuming foods containing bioactive compounds. ^Functional milk products contain bioactive components to improve metabolism, the immune system and digestion. Optimization of functional milk products can be enriched through fortification of by-products that are rarely utilized, even though they have the potential to be explored and developed as natural antioxidants. The addition of dragon fruit skin extract at a concentration of 5% produces the best red bean sprout milk yogurt with pH (4.34), viscosity (4006 mPas), antioxidants (26.17%RSA), and sensory (4.02). Yogurt drink with the addition of 3% mangosteen skin extract has a viscosity value of 74.65 cp, lightness (L) 79.89, redness (a *) 0.14 yellowness (b *) 2.32, organoleptic test of viscosity with a score of 2.84 (slightly thick), organoleptic test of color 3.52 (brownish white) and organoleptic test of texture with a score of 2.12 (rather smooth). Based on several research results, it shows that the potential of local by-products can provide significant results for use in functional milk products. Keywords: by-product- functional dairy products Share Link | Plain Format | Corresponding Author (Aju Tjatur Nugroho Krisnaningsih)

21	Animal Products and Sustainable Post-Harvest Technology	ABS-41
NUTRITIONAL CONTENT OF CHICKEN EGG FLOUR FED WITH MIANA FLOUR (Coleus scutellarioides (L.) Jein Rinny Leke1*, Erwin Wantasen2, Nio Sang Ai3, Ratna Siahaan4, Florencia Sompie5, Charles Kaunang6 and Hengki Kiroh7 Sam Ratulangi University Abstract Eggs are a source of almost perfect. Eggs consist of three The main component is the eggshell (shell) with membranes, egg whites and yolks. high moisture content, fat and protein in eggs, making eggs good bacterial growth medium up to life keep it short enough. The method used in the drying process is a method to remove or remove part of the water from a food ingredient by evaporation using heat energy. Drying egg flour is carried out by the method of oven drying. Based on the results of the analysis of oven egg flour, the egg protein content R1 41,25 % ,R2 41,29 % ,R3 41,21 % ,R4 41,35 % , Fat content R1 30,96 %, R2 30,50 %, R3 30,04 % dan R4 28,98 %. Carbohydrate Rate R1 11,20 % ,R2 11,25 % ,R3 12,20 % ,R4 12,35 % Water activities (Aw) 0.5 + 0 1 and pH 7.6 + 0 . Emulsion capacity (%) 54.1 + 1.2. Nutrition conclusion chicken egg flour protein, fat, carbohydrates, water activity and PH provide good quality. Keywords: Egg Flour, Miana Chicken Feed, Nutrition. Share Link | Plain Format | Corresponding Author (Jein Rinny Leke)

22	Animal Products and Sustainable Post-Harvest Technology	ABS-46
Enzymatic Production of Sheepskin Protein and Analysis of the Physico-Chemical Characteristics Dita Prameswari Trenggono Putri (a*), Mohammad Zainal Abidin (a), Nanung Agus Fitriyanto (a) and Yuny Erwanto (a) a) Faculty of Animal Science, Universitas Gadjah Mada, Jalan Fauna no 3, Bulaksumur, UGM, Yogyakarta, Indonesia, 55281. Abstract Increased animal consumption has led to increased slaughter of livestock, including sheep. It also increases the number of livestock by-products, such as livestock skin, which, if not utilized thoroughly, can cause waste that negatively impacts the environment. This study aimed to utilize sheep by-products and determine the characteristics of Indonesian local sheep skin protein extracted using bromelain. Sheepskin was cleaned from most of the hair, residual fat, and meat, cut into small pieces, prepped using NaOH soaking, and continued soaking using CH3COOH solution and 0.5% (w/w) bromelain enzyme. After extraction, precipitation, dialysis, and freeze-drying process followed. Characterization of the protein included SDS-PAGE, FTIR, DSC, pH, and viscosity tests. The result of the SDS-PAGE test is that a protein band is in the range of 100 to 150 kDa. FTIR test results show there are five amides. DSC test results have two peaks, the first at 80C and the second at 159.12C. The results of the viscosity and pH tests are related- the more acidic the pH of the solvent, the smaller the viscosity value of the sample solution. The characteristics of the sample show the properties of collagen protein, but the identification of the specific type of protein contained in the sample needs to be done more deeply. Keywords: Protein, Sheepskin, Physico-Chemical characteristic, Bromelain, Extraction, Production Share Link | Plain Format | Corresponding Author (Dita Prameswari Trenggono Putri)

23	Animal Products and Sustainable Post-Harvest Technology	ABS-51
Effect of adding duck feet gelatin as a binder on the physical and chemical quality of duck meatballs Meity Sompie*, Jola Londok, and Ingriet Lumentah Faculty of Animal Husbandry, Universitas Sam Ratulangi, Manado 95115, Indonesia Abstract This study has been conducted with the aim of examining the effect of adding different concentrations of duck feet gelatin on the physicochemical properties of duck meatballs. This study was carried out at the Laboratory of Animal Products Technology, Faculty of Animal Husbandry, Sam Ratulangi UniversityThe main ingredients used in this study are duck feet gelatin and meat duck. This study used a Completely Randomized Design (CRD) 4x4 (To = 0%, T1 = 2% T2 = 4% and T3 = 6%), each treatment was repeated 4 times. The research variables observed were water holding capacity, cooking loss, tenderness, pH value, water content and protein content of duck meatballs. The results showed that the addition of duck feet gelatin had a significantly different effect (P<0.05) on the water holding capacity, cooking loss, tenderness and protein content of duck meatballs, but there was no significant difference (P>0.05) on the pH value and water content of duck meatballs gelatin. The conclusion obtained in this study is the adding of 4% duck feet gelatin on the dough of meatballs as a binder produced a good quality of duck meatballs. Keywords: Binder, Duck feet gelatin, Meatballs Share Link | Plain Format | Corresponding Author (Meity Sompie)

24	Animal Products and Sustainable Post-Harvest Technology	ABS-55
Potential of Herbal Immune Probiotics as Immunity Boosters in KUB 2 Chickens Nurul Humaidah, a) Muhammad Farid Wadjdi, a) Sri Susilowati, a) Inggit Kentjonowaty a) Universitas Islam of Malang. Jl. Mayjen Haryono 193 Malang Indonesia 65144 Abstract Herbal immune probiotics are additive feed given to increase immunity. The aim of the research was to evaluate the immune potential that emerged in KUB 2 chickens that were given herbal immune probiotics. The research material is KUB 2 Chicken, a probiotic and herbal. Treatment is administration of herbal immune probiotics through drinking water. There were 4 treatments and 5 replications with 5 chickens in each replication. Treatment includes: P0= control, P1= 2 ml/L of herbal probiotics, P2=4ml, P3=6 ml. The research method is experimental. The research design used was a completely randomized design. Data analysis used ANOVA. The research variables were the number of erythrocytes, Ig G and Ig E. The results of the study showed that giving immune herbal probiotics to KUB 2 chickens had no effect (P<0,05) on the number of erythrocytes and Ig E but had an effect (P>0,05) to Ig G. The average amount of Ig G (mg/mL) is P0=4.66, P1=10.22, P2=14.43, P3=16.89. The conclusion is that giving Immune probiotics can increase Immune G in KUB Chickens. Keywords: probiotics, immunity, herbs, KUB 2 chicken Share Link | Plain Format | Corresponding Author (Nurul Humaidah)

25	Animal Products and Sustainable Post-Harvest Technology	ABS-56
A Comparative Study of Milk and Yogurt Ratios in Probiotic Ice Cream Production Lilik Eka Radiati(a*), Ahmad Khoirul Umam(a), Zia ul Rahman Fithron (a), Andika Arifsyah(a) and Johan Wirawan(a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *lilik.eka[at]ub.ac.id Abstract This study aimed to investigate the impact of different milk-yogurt ratios on the physicochemical properties of probiotic ice cream. Probiotic ice cream samples were prepared using Lactobacillus bulgaricus and Streptococcus thermophilus as starter cultures. The treatments included various ratios of milk and yoghurt: P0 (80% milk, 0% yoghurt), P1 (75% milk, 5% yoghurt), P2 (70% milk, 10% yoghurt), and P3 (65% milk, 15% yoghurt). Results showed that the milk-yoghurt ratio significantly impacted water content, colour (a*, b*, l), pH, and titratable acidity. Increasing the yoghurt ratio generally led to higher water content, viscosity and overrun, lower pH and density, increased redness (a*), and lightness (l). However, the effect on yellowness (b*) was not significant. In conclusion, our study has shown that adding yoghurt can enhance probiotic ice cream^s physical and chemical attributes, potentially improving its sensory qualities and nutritional value. These findings could pave the way for developing healthier and more appealing probiotic ice cream products. Further research is necessary to assess the effects of probiotic ice cream on individuals with lactose intolerance. Keywords: Probiotic, Functional Food, Ice cream Share Link | Plain Format | Corresponding Author (Lilik Eka Radiati)

26	Animal Products and Sustainable Post-Harvest Technology	ABS-57
The Effect Of Administration Of Immune Herbal Probiotics On Oxidative Stress Profile and Performance Of KUB 2 Chickens Haris Seno Ali, a) Nurul Humaidah, a) Umi Kalsum, a) Universitas Islam Malang, Jl. Mayjen Haryono No. 193 Malang Indonesia 65144 Abstract Probiotic herbal immunity is a nutrient that contains probiotics and enzymes. The aim of the research was to evaluate the effect of providing immune herbal probiotics on the oxidative stress profile and performance of KUB 2 chickens. The research material was 192 KUB-2 roosters aged 21 days and immune herbal probiotics. The research method is an experimental method. There were 4 treatments with 4 repetitions. Treatment were P0 = Control, P1 = 2 ml/L immune herbal probiotics, P2 = 4 ml/L, P3 = 6 ml/L. The variables measured were Oxidative Stress Profile and nett gain. The data were analyzed using the ANOVA. The results showed that administration of immune herbal probiotics had a very significant effect (P<0.01) on SOD activity and MDA levels and had no significant effect (P>0.05) on Nett Gain. The average value of SOD activity (mmol/L) was P0 : 47.00b, P1 : 14.33a P2 : 31.72ab and P3 : 43.94bc. The average MDA levels (mmol/L) P0 : 6.28a, P1 : 48.69a, P2 : 41.11b and P3 : 14.51b. The conclusion of the research was that giving Immune Herbal Probiotics had an effect on SOD activity and MDA levels in KUB 2 Chickens. Keywords: Chicken KUB-2- sod-mda-Immune-herbs Share Link | Plain Format | Corresponding Author (Haris Seno Ali)

27	Animal Products and Sustainable Post-Harvest Technology	ABS-58
Lipid Profile of Chicken Sausage Made from Pre-emulsified Vegetable Oils Dicky Tri Utama*, Jajang Gumilar, Andry Pratama Department of Animal Product Technology, Faculty of Animal Husbandry, Universitas Padjadjaran, Sumedang, Indonesia, 45363 *d.utama[at]unpad.ac.id Abstract The objective of this study was to evaluate the use of different pre-emulsified vegetable oils made from red palm olein (RPO) mixed with canola oil (RPO-CAN) or olive oil (RPO-OLV) in proportions of 30:70 (w/w) and compared to margarine (MRG) and beef tallow (BTL) on the quality of chicken sausage. Soy protein isolate, emulsifier E471, and inulin were selected as emulsion stabilizers. The potential use of pre-emulsified vegetable oils in chicken sausages as a substitute for animal fat was evaluated. The sausage was made with 60% (w/w) ground chicken breast, 20% (w/w) fat including beef tallow, margarine and pre-emulsified vegetable oils as treatment, and 20% (w/w) icy water. The use of pre-emulsified vegetable oils reduced fat content and increased the yield of chicken sausage, regardless the differences on vegetable oil mixtures. The proportion of saturated fatty acids and the n6 to n3 ratio decreased in comparison with beef tallow. An emulsion of RPO-CAN was found to have better fatty acid profile and yield in chicken sausage than RPO-OLV, MRG and BTL and could be potentially used to substitute animal fat. Keywords: Canola oil- Fatty acid- Margarine- Meat product- Red palm olein- Olive oil Share Link | Plain Format | Corresponding Author (Dicky Tri Utama)

28	Animal Products and Sustainable Post-Harvest Technology	ABS-61
Rapid Detection Method of Carbosulfan Pesticide in Apis mellifera Multiflower Honey Integrated with Internet of Things Maulidya Zahrina Qolby1*, Muhammad Panji Sofyan1, Indah Rizki Kurniawati3, Abdul Manab2 1. Student, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia 2. Lecturer, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia 3. Student, Faculty of Agriculture, Universitas Brawijaya, Malang 65145, Indonesia Abstract Honey is a bee product that potentially to be developed in Indonesia. Cultivated honey in Indonesia reaches 5,000 tons per year. One of the factors that can reduce the quality of honey is pesticide contamination that can affect honey quality and endanger human health. This study aims to develop rapid detection method of pesticide contamination in honey usingmechanism of inhibition of coloured acetylcholinesterase enzyme based on paperstrip and determine metabolomic changes factor. This rapid detection method uses AS7263 spectral sensor for accuracy of pesticide levels and is integrated with the Internet of Things. The research was conducted in laboratory experiments by testing the absorbance of pesticide levels, linearity of sensor detection, and metabolomic profiles. The results showed that the linearity of sensor readings was 96.24% with absorbance testing of pesticide levels using the UV-Vis spectrophotometer method. While metabolomic changes in the sample are not significant because the derivative compounds produced remain exogenous. This method is expected to be a solution to overcome the limitations of pesticide detection methods in honey, to be a novel method of testing contaminants in honey products that are rapid and accurate in an effort to escalate food safety in Indonesia. Keywords: AS7263 Spectral Sensor, Internet of Things, Multiflower Honey, Pesticide Share Link | Plain Format | Corresponding Author (Maulidya Zahrina Qolby)

29	Animal Products and Sustainable Post-Harvest Technology	ABS-63
Physical Quality of Crossbred Duck Meat Fed Fermented Water Hyacinth Adelina Ari Hamiyanti, Leonardo Agustyan, Anik Aprilia, Nabila Putri Faradiba, Eka Nurwahyuni Faculty of Animal Science, Universitas Brawijaya Abstract This study aims to determine the effect of water hyacinth fermentation on the physical quality of the meat of Crossbred Duck. The material used in this study was 100 Crossbred duck obtained from UPT. PT. HMT. Kediri. The research method used a Completely Randomized Design (CRD) experimental design. The treatments used were 4 treatments with 5 replications so that 20 experimental units were obtained, each experimental unit containing 5 samples and given the following feed treatments: P0 = 100% Basal Feed, P1 = 80% basal feed + 20% water hyacinth fermentation P2 = 60% basal feed + 40% water hyacinth fermentation, P1 = 40% basal feed + 60% water hyacinth fermentation. The results showed that the provision of water hyacinth fermentation with basal feed had a significant effect (P<0.05) on water holding capacity and meat tenderness, without giving a significant difference (P>0.05) on pH and cooking loss. The test results gave pH levels in sequence, namely P0 = 7.92, P1 = 8.07, P2 = 7.99 and P3 = 7.84. Water holding capacity in sequence P0 (12.92+-3.63), P1 (10.66+-1.60), P2 (9.87+-4.78), and P3 (5.77+-4.48). Cooking loss in sequence, namely P0 = 21.13%, P1 = 21.94%, P2 = 24.43% and P3 = 25.36%. The tenderness in sequence is P0 = 13.00 mm/g/10s, P1 = 12.51 mm/g/10s, P2 = 12.16 mm/g/10s and P3 = 11.93 mm/g/10s. The conclusion of this study is that the best use of fermented water hyacinth feed is in P1 (basal feed + 20% fermented water hyacinth) because it produces the best water holding capacity and tenderness. Keywords: water hyacinth, pH, water holding capacity, cooking loss, tenderness Share Link | Plain Format | Corresponding Author (Adelina Ari Hamiyanti)

30	Animal Products and Sustainable Post-Harvest Technology	ABS-65
ANALYSIS EFFECT OF ADDING MANGOSTEEN PEEL XANTHONE EXTRACT (GARCINIA MANGOSTANA) ON THE QUALITY OF BEEF SMART COATING Dafa Rafi^ul Laudza1, M. Seftian Afandi1, Ardian Yhosi Setyawan1, M. Zinedine Al Khawarizmy2, Abdul Manab2* 1Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia 2 faculty of engineering, University Brawijaya, Malang 65145, Indonesia Abstract Abstract. Beef is a healthy source of protein for consumers, but beef has a problem that is perishable. The problem of decreased beef freshness is related to several processes such as the activity of various types of bacteria, biochemical reactions, and lipid oxidation. Discoloration, damage to amino acids and fatty acids are the results of the decay process characterized by the formation of various volatile organic compounds, especially biogenic amines. Many cases in the community are sick because they consume beef that has decreased in quality, thus increasing attention to improve beef quality control such as smart coating packaging. On the other hand, mangosteen peel contains xanthone compounds that have strong antimicrobial and antioxidant activities and can be utilized as a packaging material in beef smart coatings. The purpose of this study was to determine the effect of mangosteen peel xanthone extract on beef smart coating in terms of antioxidant activity, antimicrobial activity and pH value. The experimental design used was a completely randomized design (RAL). The results showed that the addition of mangosteen rind xanthone extract had a very significant effect on antimicrobial and antioxidant smart coatings, but had no effect on the pH value of smart coatings. the addition of xanthone extract also had a significant effect on the pH value of smart coatings. Keywords: Keywords: smart coating, beef, xanthone, antioxidant and antimicrobial. Share Link | Plain Format | Corresponding Author (Dafa Rafiul Laudza)

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