Quality and Proportion of Bali Bull Spermatozoa Using The Percoll Density Gradient Centrifugation Method on Different Gradients with Andromed Diluent
Aditiya Wahyudi(a*), Aulia Puspita Anugra Yekti(b*), and Trinil Susilawati(b*)

a) Student at Fakultas Peternakan Universitas Brawijaya jalan Veteran No.10-11, Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur 65145
b) Lecturer at Fakultas Peternakan Universitas Brawijaya jalan Veteran No.10-11, Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur 65145
Email: aditiyawahyudi[at]student.ub.ac.id, auliapay[at]ub.ac.id,
and tsusilawati[at]ub.ac.id

Abstract

This study aims to determine the quality and proportion of Bali Bull semen used the Percoll density gradient centrifugation (PDGC) method at different gradients with Andromed diluent. The study was conducted at Singosari Artificial Insemination Center from December 2023 to February 2024. The experimental method used was a Randomized Group Design with sexed semen PDGC method treatment. The treatment in the study was T1: 10 Gradient, T2: 5 Gradient, and T3: 3 Gradient with 10 replications. Statistical analysis showed highly significant differences (P<0.01) in differences gradient treatments in the lower layer of individual motility. The results showed that the percentage of motility resulting from sexing in the lower layer obtained the highest value in T1 77, 86 %,while in the upper layer the highest percentage of motility was obtained in T3 which is 61,72% Meanwhile, in terms of viability, abnormalities, total motile spermatozoa and concentration, there were no differences (P>0.05) in differences gradient treatments. While the proportion of spermatozoa in sexed X and Y, T1=77.6% and 84,8%- T2=75% and 81,8%- T3=75,8% and 79,1%. In conclusion, the quality of sexed semen is categorized as good, in terms of the parameters of motility, concentration and total motile spermatozoa.

Keywords: Andromed, bali cattle, percoll density gradien centrifugation, sexing, quality.

Topic: Animal Breeding and Reproduction