The Quality and Proportion of Spermatozoa X and Y in Sexed Semen of Bali Bull with the Percoll Density Gradient Centrifugation Method with Various Gradients using Egg Yolk Tris Aminomethane Diluent Nanda Ayu Rahmawati (a*), Aulia Puspita Anugra Yekti (b), and Trinil Susilawati (b)
a) Student of Faculty of Animal Science, Universitas Brawijaya
Jalan Veteran No.10-11, Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur 65145
*nandaayur[at]student.ub.ac.id
b) Lecturer of Faculty of Animal Science, Universitas Brawijaya
Jalan Veteran No.10-11, Ketawanggede, Kec. Lowokwaru, Kota Malang, Jawa Timur 65145
Abstract
This research aims to determine the quality and proportion of X and Y spermatozoa in sexed semen that separated using the Percoll Density Gradient Centrifugation (PDGC) method with various gradients using egg yolk tris aminomethane diluent. The study was conducted at the Singosari Artificial Insemination Center from December 2023 to February 2024. The material of the study was the semen of 1 bali bull with individual motility ≥- 70%. The experimental design used a Randomized Group Design with the sexed semen PDGC method treatment: T1 = 10 gradients, T2 = 5 gradients, and T3 = 3 gradients, each with 10 replications. The analysis results showed that the gradient variations had a significant impact on the individual motility of the lower layer of sexed semen (P<0.05). Conversely, gradient variance did not affect of viability, abnormality, concentration, total motile spermatozoa, and individual motility of the upper layer of sexed semen (P>0.05). Regarding the proportion of sexed X and Y spermatozoa, the results were as follows: T1 = 85.40% and 79.80%- T2 = 81.20% and 77.90%- T3 = 75.10% and 79.90%. In conclusion, sexed semen using the PDGC method with varied gradients is suitable for the freezing process due to its high quality.
Keywords: Bali Bull, Percoll Density Gradient Centrifugation, Sexed Semen, Tris Aminomethane + 20% Egg Yolk Diluent
