Quality and Proportion of X and Y Spermatozoa Using Different Sexed Methods in Friesian Holstein Cattle Firlia Safa (a*), Amir Firdaus (a), Putri Utami (b), Aulia Puspita Anugra Yekti (c), Achadiah Rachmawati (c), Trinil Susilawati (c)
a) Post Graduate Program Faculty of Animal Science, Brawijaya University, Malang, Indonesia, 65145
*safa.firlia[at]gmail.com
b) Doctoral Program Faculty of Animal Science, Brawijaya University
c) Lecturer of Faculty of Animal Science, Brawijaya University
Abstract
Sexing process can damage the spermatozoa membrane, which causes a decrease in the quality of the spermatozoa, so an appropriate method is needed to protect the spermatozoa membrane and maintain its quality. This study aimed to determine the quality of frozen sexed semen using Percoll Density Gradient Centrifugation (PDGC) method from Singosari Artificial Insemination Center and frozen sexed semen using the Albumin Sedimentation (AS) method from Lembang Artificial Insemination Center. Each type of sample was repeated ten times. The variables used were individual motility, viability, abnormality, concentration and total motile spermatozoa (TMS). Data analysis used in this research was a variety of Analysis of Variance, Duncan Multiple Range Test and Chi Square Test. The results of unsexed frozen semen, sexed frozen semen using PDGC method, and sexed frozen semen using AS method showed that individual motility values were (46.0, 41.1, 40.6%), viability (66.1, 59.8, 61.4%), concentration (34.7, 28.5, 12.6 million/straw) and TMS (16.0, 11.7, 5.1 million/straw). The results showed that frozen sexed semen using the PDGC method has qualified the Indonesian National Standard so it can be distributed and feasible to be inseminated. Frozen sexed semen using the AS method on spermatozoa concentration and TMS value has not qualified for the Indonesian National Standard.
Keywords: Albumin sedimentation- Friesian holstein- Percoll density gradien centrifugation- Sexed semen- Semen quality- Spermatozoa proportion
