Identification Of IGF1 Gene Polymorphisms Using PCR-RFLP In Indigenous Goats Of East Java
Wike Andre Septian1 , Ari Ardiantoro1, Kuswati1, Muhaimin Rifa^i2, Ale1, and Suyadi1

1Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
2Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang 65145, Indonesia

Abstract

Abstract. The objective of this study was to identify insulin growth factor (IGF-1) gene polymorphism using PCR RFLP technique in order to improve growth evaluation. The insulin-like growth factor 1 (IGF1) gene is a critical factor in the growth and development of goats. It influences a range of traits, including body size, milk production, and reproductive performance. This gene was therefore identified as a potential candidate gene. Blood samples were collected from 120 local goats of East Java, namely 40 etawa goats, 40 senduro goats, and 40 peanut goats. The DNA was extracted using the Geneaid DNA Purification Kit. The IGF-1 polymorphisms were determined by the PCR-RFLP method, employing the HaeIII restriction enzyme. The primers for the 398 bp long IGF1 gene are forward 3^ TTGAACAGACAAGCCCAC and reverse 5^ CTCCCTTCTACTGCTGTG. The results demonstrated that the local goat population in East Java is in Hardy-Weinberg equilibrium. The conclusion of this study is that the IGF-1 gene in local goats in East Java is polymorphic, and this diversity information can be employed for association studies with growth traits, litter size, and twinning rates.

Keywords: IGF1 gene- Indigenous Goats- PCR-RFLP

Topic: Animal Breeding and Reproduction