Preliminary SNP detection of HSPA1A gene in Indonesian local sheep Aprilianna Putri Zahara Nafsina Luvita Sari1 and Alek Ibrahim2
1 Faculty of Animal Husbandry, Padjadjaran University, Sumedang, Indonesia
2 Research Center of Animal Husbandry, Organization of Food and Agriculture, National Research and Innovation Agency (BRIN)
Abstract
Heat shock proteins (HSPs) are a collection of proteins that promote thermotolerance in cells and protect against environmental stress. This study was a preliminary analysis to identify the nucleotide variant within the partial HSPA1A gene in several Indonesian sheep breeds. The blood samples were collected from five breeds of Indonesian sheep (Sapudi, Batur, Sakub, Wonosobo, and Garut) to be analyzed and amplified using a specific primer. As a result, based on the sequence alignment and direct observation of the electropherograms, three variants were found, namely SNP c.268A>G, c.503A>G, and c.733A>G. All SNPs were categorized as synonymous mutations since they did not alter the amino acid. The genotyping of samples showed two types of genotypes from each SNP. In conclusion, our study suggests exploring other regions of the HSPA1A gene to find candidate mutations for further genetic diversity studies.
Keywords: Heat shock protein, HSPA1A, sheep, Indonesia, SNP
