Dragon Fruit (Hilocereus undatus) Explant Growth Regeneration at Different Planting Positions and Media Composition In Vitro Rihlaeni Duha A. Baso, S.Pd (a*) Dr. Andi Ilham Latunra, M.Si (b) Dr. Eva Johannes, M.Si (c)
1.
Master student of Biology, Hasanuddin University, Makassar Indonesia
2.
Departement of Biology, Faculty o Mathematics and Natural Sciences, Hasanuddin university, Makassar Indonesia
Abstract
Dragon fruit (Hylocerus undatus) is a plant originating from Mexico, Central America, and South America. Currently the level of consumption of dragon fruit in Indonesia is getting higher, one type of dragon fruit that is favored by the public is red dragon fruit. Dragon fruit plants can be cultivated as agricultural crops that produce superior commodities and the demand is increasing from year to year, but the demand that can be met is only about 50%. Seeing the condition of providing the seeds, the nursery can be done in various ways. To obtain uniform plants in large numbers with good fruit quality, it is possible to obtain plant propagation through tissue culture. This study aimed to analyze the effect of regeneration and acclimatization of dragon fruit (Hilocereus undatus) explant growth at different planting positions and media composition in vitro. In dragon fruit varieties, synthetic hormones were added, namely BAP and NAA. This research was conducted from July to September 2021 at the Tissue Culture Laboratory, Faculty of Science and Nature, Hasanuddin University Makassar. The research method used is quantitative which shows the relationship between variables. The research approach is in the form of an experimental one that applies the principles of controlling the things that affect the course of the experiment, consisting of 9 treatments, namely: N0 (MS), N1 (BAP 2 ml/L), N2 (BAP 3 ml/L), N3 ( NAA 2 ml/L), N4 (NAA 3 ml/L), N5 (BAP 2 ml/L+NAA 2 ml/L) + N6 (BAP 2 ml/L+NAA 3 ml/L)+ N7 (BAP 3 ml/L, NAA 2 ml/L) + N8 (BAP 3 ml/L, and NAA 3 ml/L). The results showed that, N1 (BAP 2 ml/L) had a significant effect on shoot induction, while for root induction the best effects were N0 (MS) and N4 (NAA 3 ml/L).
Keywords: Hilocereus undatus. , Regeneration, BAP, NAA.
