CLONAL PROPAGATION of SANDALWOOD (Santalum album L.) THROUGH TISSUE CULTURE BY USING BRANCH SOAKED EXPLANTS Yohannes Wibisono1, Toni Herawan1, Asri Insiana Putri1, Fithry Ardhany1, and Liliek Haryjanto1
Center of Forest Biotechnology and Tree Improvement Research and Development Yogyakarta, Indonesia
Abstract
ABSTRACT
Sandalwood (Santalum album L) is well known for its enormous economic value. The high value of santalol contained in its oil, are inline with the price and quality grade of the wood products. Prior researches on genetic diversity and high oil quality producer trees have provide selected superior tress to be propagated. Our study objectives were to provide clonal propagation of selected superior-trees using tissue culture techniques. Four mother trees, named P.6.1, P.6.2, A.III.4.14, and TA.12, were selected to run the test. Shoots from branch-soaked in tap water were utilized in shoots cultures. Study showed that P.6.1 gave greatest number of shoots produced in soaking branches technique, while A.III.4.14 produced mean longest shoots. Best induction response showed by P.6.1 (90%) while in multiplication phase A.III.4.14 produced highest mean average shoots number with 24 shoots and mean longest shoots with 4.3 cm. Further study on rooting ability, using A.III.4.14 clones, showed that it greatest success rate after 3 months after induction. Acclimatization phase obtain media M1P3 (top soil + sands + fertilizer yeast+ 1 ml L-1) gave the best response to the growth of shoots and roots of sandalwood plantlets.
Keywords: Keywords: Santalum album, clones, propagation, tissue culture.
Topic: Emerging Technologies in Agricultural Production Systems
