Sequencing Analysis of IRT Gene from Iron Tolerant Rice (Oryza sativa L.) Variant in Indonesia Rizky Priambodo, Alma Fahira, Adisyahputra
Biology Study Program, Universitas Negeri Jakarta, Jalan Rawamangun Muka Raya, Jakarta, Indonesia, 13220
Abstract
Indonesia consumes rice as its primary meal, despite the fact that land for growing rice is already scarce. To boost national rice production, swamp area might be utilized for cultivation. Swamp land^s high concentration of Fe makes it difficult to utilize. Molecular analysis are needed for the quick and effective selection of rice strains that can withstand iron stress. The objective of this study is to analaysis the sequence of IRT gene as a potential marker for iron tolerant rice variant. The DNA for this study was taken from several strain of rice, including the the iron tolerant and non iron rolerant rice variant. The outcomes demonstrated that Siam Saba rice variant has a deletion mutation at position 1007 bp and an insertion mutation at position 1151 bp. Cluster analysis identified two primary groups of four rice strains, which is predicted as group of iron tolerant rice and non iron tolerant rice. Siam Saba and Siam Tanggung make up the first group, while Ciherang and INPARI 34, make up the second. Twelve SNP sites were also revealed using SNP marker analysis. The functional gene study was needed to determine the gene marker candidate related with the iron tolerant character.
Keywords: Rice, iron tolerant, IRT gene, sequencing
