Phytochemical profiles of Persea fruticosa extracts and its antimicrobial potential Tayal Punitha Ramasamy Naidu, Dr Norazah Binti Basar, Dr Syazwani Itri Binti Amran
University Technology Malaysia, Malaysia
Abstract
Antimicrobial resistance (AMR) is one of the top 10 global public health threats faced by humanity. Overuse and not being compliant in antibiotic consumption, led to the growth of drug-resistant pathogens. Medicinal plants have a continuous attraction for an effective antimicrobial agent against resistant pathogens despite the conventional drugs available for infection. Persea americana has been studied widely. But there is very limited study on Persea fruticosa species. This study will be focusing on the identification of phytomedicinal compounds of Persea fruticosa. The antimicrobial activity was tested in vitro. Qualitative analysis of the leaves of Persea fruticosa through thin layer chromatography (TLC) analysis on the petrol ether, ethyl acetate, and methanol extracts revealed the presence of a diverse range of bioactive compounds, including flavonoids and terpenoids. In order to emphasize on polar compounds, methanol extract was subjected to LC -MS analysis, which resulted with ketone, alkaloids, alcohol and amino acids. The affinity of these compounds against four target proteins, Gyrase B, Penicillin binding protein, D alanine and Dihydrofolate were assessed using molecular docking approach. Among the tested compounds, fluoren-9-one demonstrated highest binding affinity ranged from -6.7 to -7.8 kcal/mol towards all proteins. This leads to exploration of the mechanism of action of Persea fruticosa as antibacterial agent. This docking data will be further tested by microbiology testing on several bacteria. This integrative approach combining experimental and computational techniques will provide a comprehensive understanding of the extracts^ antimicrobial potential. This provides promising solution for antimicrobial resistance and minimizing the over usage of antibiotics.
Keywords: Antimicrobial resistance (AMR), antimicrobial activity, Persea fruticosa, in vitro and in silico
