Entrapment Efficiency and Morphological Study of Temulawak-loaded Nanostructured Lipid Carrier (NLC) 1Mei Lilis Suryani, 1Bambang Susilo, 2Nozomi Watanabe, 2Hiroshi Umakoshi, 1Nimatul Izza*
1 Department of Biosystems Engineering- Faculty of Agricultural Technology - Universitas Brawijaya
2 Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University
*email: izza_nimatul[at]ub.ac.id
Abstract
Nanostructured Lipid Carriers (NLCs) represent the second generation of lipid nanocarriers, succeeding Solid Lipid Nanoparticles (SLNs), and are extensively developed in the pharmaceutical field. This study investigates the entrapment efficiency of Temulawak-loaded NLCs and examines the morphology of the lipid formation. NLCs were composed of cetyl palmitate, caprylic triglycerides, and polysorbate 80, and were prepared using hot homogenization and ultrasonication methods. The results demonstrated that the NLCs had an average particle size ranging from 134 to 207 nm with a homogeneous size distribution. Zeta potential measurements confirmed the stability of all the NLC formulations. Furthermore, the entrapment efficiency of Temulawak extract in the NLCs exceeded 70%, indicating that NLCs could serve as effective carriers for bioactive compounds. Additional characterizations, including FTIR to confirm the successful entrapment of Temulawak and morphological studies using TEM, were also focal points of this research. Through these studies and characterizations, the behavior of Temulawak-loaded NLCs will be better understood, providing essential insights for designing NLCs as carriers for bioactive compounds with broad applications.
