Optimization of The Maceration Extraction Method of ^Bajakah Tampala Stems^ (Spatholobus littoralis Hassk.) Stefani*, Erryana Martati, Ahmad Zaki Mubarok
Department of Food Science and Biotechnology, Faculty of Agricultural Technology, Universitas Brawijaya, Indonesia
*Email: stefani_thp[at]student.ub.ac.id
Abstract
^Bajakah tampala^, a stem from a tree of ^Bajakah Tampala^ (Spatholobus littoralis Hassk.) is rich in bioactive compounds such as alkaloids, flavonoids, tannins and saponins. This research aims to determine the optimum temperature and the ratio of material to solvent in the maceration process of Bajakah Tampala using a water bath shaker. Optimization of the extraction process was carried out using Response Surface Methodology in Design Expert 12 software with a Central-Composite Design which resulted in 13 runs. This research used two factors, namely temperature (with a lower limit of 50oC and an upper limit of 70oC) and the ratio of material to solvent (with a lower limit of 1:20 and an upper limit of 1:40). The maximum expected response results are total phenols, total flavonoids and antioxidant activity. The experimental results show that the optimal conditions for extracting bajakah tampala using a water bath shaker are at a temperature of 62.3oC with a material to solvent ratio of 1: 33.5 - which produces total phenols of 5.989 mg GAE/g- total flavonoids of 4.467 mg QE/g- and antioxidant activity of 45.46%.
