Antioxidant and Anti-Breast Cancer Herbal from Uncaria Gambir Roxb Leaves: In Silico & In Vitro Study Dodi Iskandar, Rollando, Susana, Y. Erning Indrastuti
Politeknik Negeri Pontianak
Abstract
Uncaria gambir Roxb (URG) is one of the plants from West Kalimantan which has been predicted to contain antioxidant and anti-breast cancer substances is UGR. This study aims to test the antioxidant and anti-breast cancer potential. UGR leaves are extracted by infudation method using water for 15 minutes with 4 repetitions at a temperature 70oC. 96.2351 grams (29.979%) of the thick extract was obtained from a sample weight of 321 grams dry UGR leaves. In vitro antioxidant assay of the extract was investigated using 2,2-diphenylpicrylhydrazyl (DPPH) with positive control using quercetin and ascorbic acid, and the Ferric Reducing Antioxidant Power (FRAP) method. In silico screening shows that the biological agents in UGR have the potential as TP53 expression enhancer, antioxidant, anticarcinogenic, chemopreventive, and free radical scavenger. The antioxidant bioassay results showed IC50 values of 81.21 μ-g/mL, 73.39 μ-g/mL, and 9.17 μ-g/mL in DPPH for extract samples with positive control quercetin, sample extracts with positive control Vitamin C and Vitamin C with positive control quercetin, respectively. Meanwhile, the antioxidant activity of extract samples with the FRAP method showed value of 66,05 μ-g/mL. Meanwhile, anticancer bioassay result showed that UGR leaves extract with water solvent had the strong potential to inhibit 4T1 cells with IC50 87.71 μ-g/mL
Keywords: Uncaria gambir Roxb, Antioxidant, Anti-Breast Cancer
