Comparring Reactive Oxygen Species and Superoxide Dismutase 3 of Biosmart and Safe Bus and Reguler Bus Passengers Ai Devi Nuraeni1,a), Awal Prasetyo2, Udadi Sadhana3, Agus Subagio4, Susilo Adi Widyanto5, Arlita Leniseptaria Antari6, Stefan Arman7, Anthony Steven Hambali8, Soerjanto Tjahjono9, Saras Pujowati1, Liftia Layyinatus Syifa1, Muflihatul Muniroh2, Neni Susilaningsih2
1Postgraduate Student - Masters in Biomedical Science, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
2Department of Biomedical Science, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
3Department of Pathology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
4Departement of Physics, Faculty of Science and Mathematics, Diponegoro University, Semarang, Indonesia 5Department of Mechanical Engineering, Faculty of Engineering, Diponegoro University, Semarang, Indonesia 6Department of Microbiology, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
7Laksana Karoseri, Semarang, Indonesia
8PO Sumber Alam, Purworejo, Indonesia
9Chairman of Komite Nasional Keselamatan Transportasi (KNKT)
Abstract
Abstract. Bus are the most popular land transportation among the people. Biosmart and safe bus apply the principle that thebus compartment which is a biological environment must be in a balanced state according to the disease triangle concept that is influenced by the host, in this case is the passenger, the agent causing the infection is a virus and the environment is the roomin the bus cabin.
The aim of the present study is to analyze the differences in ROS and SOD3 levels of Biosmart and safe bus passengers and reguler bus passengers.
The research is a non-blinded randomized control trial with pre-posttest design. Eighty passengers were randomly separated into two groups. control group 40 uses reguler bus, and intervention group uses Biosmart and safe bus 40. Nasal wash was taken by ENT specialists, and the levels of Sinonasal ROS and SOD3 were interpreted by ELISA. The data were analyzed by using Mann Whitney, Wilcoxon, Independent t-test and Paired t-test.
The results showed that the average level of SOD3 in the sample of Biosmart nd safe bus passengers samples were higher than in reguler bus passengers. The difference in ROS levels in the treatment group was lower than in the control group which was stated to have a significant effect with a p value last than 0,001 in both groups.
Conclusion
Based on the presentatin of the data and discussion in the previous chapter, it can be concluded from this study that there is a statistically significant difference in ROS levels for Biosmart and safe bus passengers after the trip. The passenger group that was given the Biosmart and safe bus treatment showed lower ROS levels compared to the reguler bus and there was also a statistically significant difference in the SOD3 levels of the Biosmart and safe bus passengers after the trip.The Biosmart and safe bus passengers group had higher SOD3 levels compared to the reguler bus.
