IDENTIFICATION OF PIROXICAM AND MEFENAMIC ACID IN JAMU PEGAL LINU USING SPECTROPHOTOMETRY UV-VIS COMBINED WITH CHEMOMETRIC Triyadi Hendra Wijaya, Beti Pudyastuti, Nia Kurnia Sholihat, Rehana, Marwa Fathin Azizah
Jenderal Soedirman University
Abstract
Background: Piroxicam and mefenamic acid has been reported as a medicinal chemical that is added to jamu pegal linu. Several methods that have been used to analyze or detect chemical adulteration on jamu use separation as basic concept, which requires substantial times. This study aimed to develop a combination method between spectrophotometry UVVis and chemometric to analyze adulterated traditional medicine.
Methods: This experimental study used spectrophotometry UV-Vis combined with chemometric. Analysis using UV-Vis spectrophotometry was carried out with UV Analyst software with the aim of obtaining absorbance results at a wavelength of 200 - 400 nm. Chemometric analysis, PCA, used Minitab 2.1 at the wavelength where piroxicam and mefenamic acid showed their peak at 267 - 385 nm.
Result: The result of PCA analyze showed good differentiation. PC1 and PC2 can represent 99% of the data variance. Jamu pegal linu in Purwokerto do not contain piroxicam and mefenamic acid.
Conclusion: Based on the result of PCA analysis, combination method between
spectrophotometry UV-Vis and chemometric can be used to identify piroxicam and mefenamic acid on jamu pegal linu. Jamu pegal linu in Purwokerto do not contain piroxicam and mefenamic acid.
Keywords: Piroxicam, Mefenamic Acid, UV-Vis Spectrophotometry, Principal Component Analysis
