Antibacterial Activity of Clove Medicinal Plants (Bischofia javanica Blume) Against Cell Damage of Staphylococcus aureus and Salmonella enterica Idramsa Idramsa (a*), Hendro Pranoto(a), Uswatun Hasanah (a)
Faculty Mathematics and Natural Sciences, Universitas Negeri Medan, Jl. Willem Iskandar Psr V-Medan Estate, Medan 20221, Indonesia.
idramsa[at]unimed.ac.id
Abstract
The development of clinical microbial strains that are resistant to one or more antibiotics has become a serious problem that must be addressed immediately. This shows that in the last decade, the demand for new antibiotic substances has increased considerably. The existence of this phenomenon is increasingly encouraging the importance and the need for research to search for new compounds that have the potential as antibacterial. Plants have a variety of secondary metabolites that can be used as natural medicinal ingredients, one of which is by utilizing plant materials such as cingkam (Bischofia javanica Blume). This study aimed to isolate secondary metabolites and to test the antibacterial activity of cingkam bark extracts. Extraction of secondary metabolites using the maceration method with methanol, ethyl acetate and hexane as solvents. The antibacterial activity test was carried out using the disc diffusion method. Determination of the minimum inhibitory concentration (MIC) and minimum killing concentration (MBC) in vitro by micro dilution method against pathogenic bacteria, namely Salmonella enterica ATCC 14028 and Staphylococcus aureus ATCC 25923.
The results of the research that have been carried out are known to have antibacterial activity against Salmonella enterica and Staphylococcus aureus. The extraction that produced the strongest antibacterial activity was the extraction technique with methanol as a solvent on Salmonella enterica bacteria. In Staphyloccus aureus, the extraction technique with ethyl acetate solvent produces the strongest antibacterial activity.
Cingkam bark extracts has antibacterial activity against Salmonella enterica and Staphylococcus aureus.
