Anti Elastase and Anti Tyrosinase Activities from The Pericarps Extract from Garcinia tetandra Pierre
a,b Neneng Siti Silfi Ambarwati, c,Berna Elya, d,Yesi Demiaty, e,Agnes Nabela, e,Yulia Triyani

a Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Pulogadung, Jakarta Timur, DKI Jakarta, 13220, Indonesia b Research Center for Cosmetics, Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Pulogadung, Jakarta Timur, DKI Jakarta, 13220, Indonesia
c Department of Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia,
Depok, 16424 West Java, Indonesia
dDepartment of Phytochemistry, Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia
e Department of Pharmaceutical Biology, Faculty of Pharmacy and Science, Muhammadiyah University of Prof. Dr. HAMKA, Jakarta, 13460, Indonesia

Abstract

Skin aging can be inhibited by reducing the action of the proteinase elastase enzyme and hyperpigmentation can be treated by inhibiting tyrosinase activity. The current study aimed to assess anti elastase and anti tyrosinase from Garcinia tetandra Pierre and investigate its flavonoid constituents. The extraction used maceration, with the ethyl acetate extract yielding 6.12 percent and the n hexane extract is 8.64 percent. In a series of in vitro assays, anti elastase (porcine pancreatic elastase) and anti tyrosinase (mushroom lyophilized powder tyrosinase) activities of various extracts of pericarps were evaluated. The assays used by microplate spectrophotometric method at a wavelength of 405 nm (for anti elastase assay) and 490 nm (for anti tyrosinase assay). The flavonoid content of the constituents was carried out on the extracts by AlCl3 method and measured using at 425 nm UV visible spectrophotometer. According to the findings, ethyl acetate extracts displayed the highest elastase inhibition (79.91) at 100 ppm and total flavonoid content 0.07 percent quercetin equivalent. The n hexane extract elastase inhibitory activity has resulted (73.78) better than quercetin as a positive control (65.97). According to the findings, n hexane extracts displayed the highest tyrosinase inhibition (53.83) at 100 ppm and total flavonoid content (1.27 percent quercetin equivalent), even though the result is lower than the kojic acid as a positive control (65.07). The flavonoid and phenolic acids can help with anti elastase inhibition activity. Implicating the research are the extracts for healthy and beauty, like skin protection, anti aging and product development potential.

Keywords: anti tyrosinase, anti elastase, Garcinia tetandra Pierre, pericarps

Topic: Chemistry