Comparative Production of Protein Hydrolysate Using LSF and SSF Methods from Chicken Feather Waste by Isolate B-9-6: A Study to Determine the Most Effective Method
Mulyono (a*), Alinil Masruroh (b), Gita Fitria (c), Dian Herasari (d)

Department of Chemistry, FMIPA Universitas Lampung
Jl. Soemantri Brodjonegoro No.1, Bandar Lampung 35145, Indonesia
*mulyono[at]fmipa.unila.ac.id

Abstract

This study evaluated protein hydrolysate production from chicken feather waste using isolate B-9-6. Protein hydrolysates were produced using liquid (LSF) and solid (SSF) fermentation techniques. A UV-Vis spectrophotometer was used to conduct the protein hydrolysate assay stages, assess the degradation of the percentage of chicken feather waste, and ferment the waste for 12 days. SEM analysis, amino acid content, protein hydrolysate content, and proteolytic activity were also included in this study. The results of previous studies showed that 70% was the optimal degradation rate for the chicken feather waste using the LSF method. The LSF approach yielded the optimal amino acid content concentration at 0.93 U/mL, while the SSF method yielded the highest keratinase proteolytic activity assay findings at 0.93 U/mL.

Keywords: Chicken feather- LSF- SSF, Keratinase

Topic: Chemistry