Immobilization of Recombinant Enzyme NcCDH (Cellobiose Dehydrogenase From Neurospora Crassa) As A Biorecognition Element For Lactose Biosensor Application In Milk
Sudarma Dita Wijayanti*, Josephine Laurentia Liem, Malya Maheswari Waluyo, Nur Ida Panca Nugrahini, Fenty Nurtyastuti E.P

Department of Food Science and Biotechnology, Faculty of Agricultural Technology, Universitas Brawijaya
*Corresponding author email : dee_ta2002[at]ub.ac.id

Abstract

Biosensor is an analytical device that combines biological components and transduction elements to detect an analyte. Biosensors have been applied in various fields, including healthcare. Glucose detection using glucose oxidase, which catalyzes the oxidation of glucose into a signal proportional to glucose concentration, is commonly used for blood sugar detection. The same principle can be applied to other types of sugars, such as lactose in milk. One enzyme that can be utilized as a bioreceptor for lactose is cellobiose dehydrogenase (CDH). The enzyme from Neurospora crassa (NcCDH) is capable of demonstrating direct electron transfer (DET), making it efficiently applicable as a third-generation biosensor. Immobilization of the NcCDH enzyme is carried out by mixing NcCDH and CS-AuNPs on a screen-printed carbon electrode. Characterization of the biosensor using electrochemical methods was conducted by measuring cyclic voltammetry and chronoamperometry using lactose substrates at various concentration as well as linearity, detection limit, and sensitivity.

Keywords: Biosensor- Electrochemistry- Lactose

Topic: Food science and biotechnology