Evaluation of Antioxidant Activity and Stability of Temulawak-incorporated Nanostructured Lipid Carriers (NLC)
1Renasya Athira Firzana, 1Inggit Kresna Maharsih, 2Hiroshi Umakoshi, 2Nozomi Watanabe, 1Nimatul Izza*

1 Bioprocess Engineering Study Program, Faculty of Agricultural Technology, Universitas Brawijaya, Malang-INDONESIA

2 Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Osaka-JAPAN

Email: izza_nimatul[at]ub.ac.id

Abstract

Nanostructured Lipid Carriers (NLCs) are advanced forms of drug delivery systems designed for enhancing bioavailability, composed of solid and liquid lipids to form a matrix structure within which the drug or active compound is dissolved or dispersed. This study investigated the impact of NLC formulations on the antioxidant activities of the loaded Temulawak (Curcuma xanthorrhiza Roxb.) extract. The research outcomes indicated a remarkable enhancement in antioxidant activities when the extract was incorporated to NLC systems. This trend was consistent across other NLC formulations, with antioxidant activity demonstrating substantial improvement compared to the pure extract. Stability testing of the NLC samples over a period of 6 months revealed a decline in antioxidant performance. The highest antioxidant activity of 53.28% was observed in the NLC with a higher liquid lipis and 3% of temulawak extract. However, after 6 months, the best stability was recorded in the same NLC formulation with 5% temulawak extract, that resulting antioxidant activity of 30.37%, and representing a percentage decrease of 27.42%. Meanwhile, The NLC with higher solid lipid and 3% temulawak extract exhibited the smallest particle size of 163.57 nm. These findings affirm the role of NLCs in boosting the efficacy of antioxidant components in Temulawak extracts while also highlighting the importance of stability in maintaining their therapeutic benefits. The study paves the way for leveraging NLCs in enhancing the therapeutic properties of natural antioxidants, potentially revolutionizing the application of bioactive compounds in medical and nutraceutical formulations.

Keywords: Antioxidant Activity-Nanostructured Lipid Carriers-NLC-Stability-Temulawak

Topic: Food science and biotechnology