Antibacterial activity and characterization of Peronema canescens Jack leaf extract nano-encapsulated loaded in chitosan under storage condition Bella Putri Maharani(a*), Hafi Luthfi Sanjaya (a), Aji Praba Baskara (a), Nanung Danar Dono (a), Bambang Ariyadi (b), and Zuprizal (a)
a)Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna 3, Bulaksumur, Yogyakarta 55281, Indonesia
b)Department of Animal Production, Faculty of Animal Science, Universitas Gadjah Mada, Jl. Fauna 3, Bulaksumur, Yogyakarta 55281, Indonesia
Abstract
Objective of this study was to determine the effects of different one-month storage conditions (at room temperature vs. in refrigerator) on the storage stability of the sungkai (Peronema canescens Jack) leaves extract, using encapsulation efficiency of phenol, particle size and distribution, zeta potential, and antibacterial activity as response criteria observed. Extraction of the sungkai leaves were performed using a maceration process to produce a thick extract for nanoencapsulation process. The leaf extract was encapsulated with chitosan using the ionic gelation method. Current study using 70% ethanol extraction showed that sungkai leaf extract contains bioactive compounds, such as phenols, flavonoids, alkaloids, steroids, and saponins. Different storage conditions did not influence antibacterial property, but both reduced zeta potential value of the nano-capsulated extract. Room temperature storage showed better particle stability as shown in the smaller particle size (91.823 nm), narrower dispersion (0.267 nm), and appeared more homogeny, than those of in-refrigerator storage. However, storage at cold temperature increased phenol nanoencapsulation efficiency by 33.017, than that of at room temperature storage. Results showed that leaf extract might be stored for one month, at room temperature or in the cold room temperature, without any adverse effect on the antibacterial property of the herbal feed additive.
Keywords: Antibacterial property, Nanoencapsulated, Peronema canescens Jack leaves, Storage stability
