Comparison of Extracellulose-producing Bacillus Isolated from the Digestive Tract of Muscovy Ducks (Cairina Moschata) and Native Chicken (Gallus Gallus Domesticus) as Probiotic Candidates for Poultry
Zaid AL Gifari (a), Khairunnisah (b), Fatimah Azh Zhahro Bagis (b), Fitri Andriani (b), Alimuddin (c,e), Muhamad Amin (d), Khairil Anwar (e), Muhammad Aidil Fitriyan Fadjar Suryadi (e),Anwar Rosyidi (e), I Wayan Wariata (e), Made Sriasih (e), Muhamad Ali (e)*

(a) Laboratory of Animal Production, Faculty of Animal Sciences, University of Mataram, Mataram, Indonesia,
(b) Graduate Program of Animal Resources, Faculty of Animal Sciences, University of Mataram, Mataram, Indonesia,
(c) Faculty of NW Mataram, Mataram, Indonesia,
(d) Fish Nutrition Research Group, Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, Indonesia,
(e) Laboratory of Biotechnology and Animal Product Processing, Faculty of Animal Sciences, University of Mataram, Mataram, Indonesia. *m_ali[at]unram.ac.id

Abstract

Probiotics have widely been used as an alternative feed supplement in poultry, contributing to enhanced productivity and the maintenance of livestock health. This study aimed to compare several extracellular cellulase-producing Bacillus spp. isolated from the digestive tract of muscovy ducks (Cairina moschata) and native chickens (Gallus gallus domesticus). A total of seven isolates were selected based on extracellular cellulase activity detected on carboxymethyl cellulose (CMC)-agar plates. Morphological, biochemical and molecular analyses were sequentially conducted to identify bacterial species. Three isolates exhibiting the best extracellular cellulase activity obtained from Muscovy Ducks (Cairina Moschata) and native chickens (Gallus gallus domesticus) were selected and identified through 16S rRNA gene sequencing. The results showed that the A1 isolate was identified as Bacillus cereus strain SA275C, the A4 isolate as Bacillus cereus strain S8 and and the A6 isolate as Bacillus subtilis strain IAM 12118. Three selected isolates showed different characteristics as probiotics for poultry, including the viability to gastrointestinal tract pH and antibacterial activity against Staphylococcus aureus. In conclusion, three selected isolates with the highest extracellular cellulase activity have potential as probiotic candidates for poultry.

Keywords: poultry, probiotics, Extracellular cellulase, Bacillus sp., 16S rRNA gene sequencing

Topic: Feed Resources and Technology