Utilization of Tofu Liquid Waste for Fungal Biomass Protein Production as an Animal Feed Supplement via Fermentation using Rhizopus oryzae and Rhizopus oligosporus Keryanti, Rintis Manfaati, Khanza Azqia Nurhaliza, Sri Puji Rahmawati
Politeknik Negeri Bandung
Abstract
Animal feed supplements are nutritious mixtures added to animal feed to improve feed nutrition according to animal needs. This animal feed supplement can be produced by adding fungal protein obtained from the fungi Rhizopus oligosporus and Rhizopus oryzae. This study aims to utilize tofu liquid waste from a tofu industry in West Bandung, which has a glucose content of 21.67% and nitrogen of 0.53%, making it a potential fermentation medium for the formation of fungal biomass protein. The fermentation process was carried out for 48 hours with pH variations of 4, 5, 6, and 7 and the addition of ammonium phosphate at concentrations of 0.25, 0.5, 1, 1.5, and 2 g/L. From the optimum conditions obtained, Kjeldahl analysis and Luff Schoorl analysis were performed. By Rhizopus oryzae, the optimum fermentation condition was at pH 5 with the addition of 1 g/L ammonium phosphate, producing 2.19 g/L of fungal biomass protein. Meanwhile by Rhizopus oligosporus, the optimum condition was at pH 6 without the addition of ammonium phosphate and resulting in a dry biomass of 1.75 g/L. The study found that the protein content in fungal protein using Rhizopus oryzae was 74.25% and Rhizopus oligosporus was 62.31%. The analysis shows that the protein content of the fungal biomass produced in this experiment is 74.25%, nearly three times higher than that of commercial supplements available on the market
