Development Of Levan Nanoparticle System As A Material For Insulin Drug Delivery Desy Kurniawati(a*), Rukman Hertadi (a), Enny Ratnaningsih(a), Neng Fisheri (b)
(a)Chemistry, Bandung Institute of Technology
Jalan Ganesha 10, Bandung 40132, Indonesia
*081383720011nanda[at]gmail.com
(b)Pharmacy School, Bandung Institute of Technology
Jalan Ganesha 10, Bandung 40132, Indonesia
Abstract
Levan is a biopolymer also known as fructan. It is a highly versatile material with various applications in industries such as medicine and chemistry. One of the uses of levan is in the field of nanotechnology, where it is used as a material to create nanoparticles. This study explores the potential of levan from the halophilic bacteria Bacillus licheniformis BK1 as a nanoparticle system for insulin delivery. FTIR was used to confirm the structure of levan. The obtained levan was acetylated and used as a nanoparticle system to immobilize insulin. The Insulin-levan nanoparticles (Lev-Ins) and Levan acetylation-insulin nanoparticles (Lac-Ins) have a size distribution in the range of 200-600 nm. Lev-Ins is stable at pH 4-7 but less stable at pH 1-3, while Lac-Ins is stable at various pH levels. The encapsulation efficiency of Lev-Ins and Lac-Ins was 78.46% and 88.30%, respectively. The results show that Lac-Ins can be used as a nanoparticle system for insulin delivery.
