DNA barcodes for Identification of Indonesian Medicinal Plant Cryptocarya pulchrinervia Risma Septi Insani, Lia Dewi Juliawaty, Yanti Rachmayanti*
Biochemistry Division, Faculty of Mathematics and Natural Sciences, Bandung Institute of Technology
Jalan Ganesha 10, Bandung 40132, Indonesia
*rachmayanti[at]itb.ac.id
Abstract
Cryptocarya is a plant genus in Indonesia that has been studied for the activity of its secondary metabolites as anticancer, antiplasmodial, and analgesic agents. One species that contains secondary metabolites with cytotoxic activity, which can be developed as an anticancer agent, is Cryptocarya pulchrinervia. The aim of this research is to develop the DNA barcode for identification of the medicinal plant Cryptocarya pulchrinervia. The leaves of this plant, which have been studied for their secondary metabolites, were used as the source of DNA. The DNA isolation was carried out using Plant Genomic DNA Extraction from Viogene. 18S rDNA, rbcL, matK, and ITS marker genes were amplified by PCR technique. The isolated DNA and gene amplicon were confirmed by agarose gel electrophoresis and a nanodrop spectrophotometer. Several marker genes were sucessfully amplified, i.e 18S rDNA (ca. 250 bp), rbcL(ca. 1500 bp), matK (ca. 450 bp), and ITS (ca. 250 bp). These genes will be used for the sequencing analysis and the developing of DNA barcode for Cryptocarya pulchrinervia.
Keywords: Cryptocarya pulchrinervia, marker genes, SNP, DNA barcode
