Anaerobic Method For Eliminating Antibiotic Residue Of Mastitis Treatment In Cow^s Milk Using Kefir Grain Umam Ahmad Khoirul1, Radiati Lilik Eka1 , Andriani Ria Dewi1, Yunita Citra Nurma2 , Ramadhaniarti Millenia Wahyu2, and Ramadhan Rizky
Universitas Brawijaya
Abstract
Appropriate technology was needed to degrade the antibiotics in waste milk after mastitis treatment. The microbiology method as an anaerobic treatment probably effectively eliminates antibiotic residues in milk because of microorganism activities. This study aimed to Eliminate Antibiotic Residue of Mastitis treatment in Cow^s Milk with an anaerobic method Using Kefir Grain. Twelve samples of Holstein cows milk with 3% of kefir grain were assigned to treatments equally: cows milk with 3 % of kefir grain without antibiotics (control) and cows milk with 3 % of kefir grain plus three levels of antibiotics: 6, 8, and 10 of Biomycin, respectively. The obtained data were statistically analyzed by Completely Randomized Design (CRD) with four treatments and three replications and continued by Duncan^s Multiple Range Test (DMRT). This research shows that the addition of 3% kefir grains indicates the elimination of antibiotics in milk with a negative result on the antibiotic residue test. A significant different effect of pH (3,98 0,03), syneresis (24.93 0.7), lactic acid bacteria viability (8.87 CFU/ml), and Total Plate Count (8,58 0,08 CFU/ml) was found on cow^s milk with 3% of kefir grain plus 6 μ- of antibiotics, except for dissolved protein (8,16 1.71).
Keywords: Antibiotic, Milk, Kefir Grain
Topic: Animal Products and Sustainable Post-Harvest Technology
