Multiplication of shoot and root induction on plantlets of Chrysanthemum (Chrysanthemum sp.) Sabiya agrihorti variety with the addition of different synthetic hormones in vitro
Nur Alfi Hidayati.S, S.Si (a*) Dr. Andi Ilham Latunra, M.Si (b) Dr.Eva Johanes, M.Si (c)

1
Master student of Biology, Hasanuddin University, Makassar, Indonesia
2
Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin
University, Makassar, Indonesia


Abstract

Ornamental plants are one of the horticultural commodities that have considerable agribusiness prospects in Indonesia. One type of ornamental plant that has high economic potential is chrysanthemum (Chrysanthemum sp.). Chrysanthemum is a popular cut flower in Indonesia, seeing the large public interest in the utilization capacity of the chrysanthemum plant, it is necessary to develop and cultivate it. However, the obstacle that is often faced is the availability of seeds, while efforts are being made to overcome it, one of which is through tissue culture techniques. This study aimed to analyze the effect of shoot and root induction multiplication on plant Chrysanthemum (Chrysanthemum sp.) plantlets of Sabiya agrihorti by adding synthetic hormones, namely BAP and NAA. This research was conducted from July to September 2021 at the Tissue Culture Laboratory, Faculty of Science and Nature, Hasanuddin University Makassar. The research method used is quantitative which shows the relationship between variables. The research approach is in the form of an experimental one that applies the principles of controlling the things that affect the course of the experiment, consisting of 9 treatments: K0 (MS), K1 (BAP 2 ml/L), K2 (BAP 4 ml/L), K3 ( NAA 2 ml/L), K4 (NAA 4 ml/L), K5 (BAP 2 ml/L+NAA 2 ml/L) + K6 (BAP 2 ml/L+NAA 4 ml/L)+ K7 (BAP 4 ml/L, NAA 2 ml/L) + K8 (BAP 4 ml/L, and NAA 4 ml/L). The results showed that K1 (BAP 2 ml/L) had a significant effect on shoot induction, while for root induction the best effects were K0 (MS) and K4 (NAA 4 ml/L).

Keywords: Chrysanthemum sp. , Multiplication, BAP, NAA.

Topic: Emerging Technologies in Agricultural Production Systems

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