Shoot Induction of Japansche Citroen (JC) (Citrus limonia Osbeck.) as Rootstock for In Vitro Micrografting
Mustika Tuwo (a,b), Tutik Kuswinanti (c*), Andi Nasruddin (c), Elis Tambaru (b)

a) Program of Agriculture, Graduate School, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km. 10, Tamalanrea, Makassar 90245, South Sulawesi, Indonesia
b) Department of Biology, Faculty of Mathematics and Natural Science, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km. 10, Tamalanrea, Makassar 90245, South Sulawesi, Indonesia
c) Department of Plant Pest and Disease, Faculty of Agriculture, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km. 10, Tamalanrea, Makassar 90245, South Sulawesi, Indonesia

Abstract

The production of pathogen-free plants can be obtained through the application of in vitro micrografting which is very important in providing certified plants to farmers so as to increase citrus productivity. The type of citrus that is commonly used as rootstock is Japansche Citroen (JC) (Citrus limonia Osbeck.) because of its compatibility with scion and good root system. The explants used in this study were seeds extracted from fresh fruit and then inoculated on MS media with the addition of the hormone 6-Benzyl-aminopurine (BAP) at a concentration of 0.5 ppm- 1 ppm- 1.5 ppm- and 2 ppm. The observation parameters used were shoot growth time and the number of shoots that appeared after treatment. The results showed that the shoots appeared the fastest and the most in the BAP treatment with a concentration of 1.5 ppm.

Keywords: Japansche citroen, BAP, seed, tissue culture

Topic: Emerging Technologies in Agricultural Production Systems