The Potency of Endophytic Bacteria Isolated from Mangrove Plants as Indole-3-acetid Acid (IAA) Producers with the Addition of L-Tryptophan
Agustina Monalisa Tangapo (a,b,*), Susan Marlein Mambu (a)

(a) Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, Indonesia.
*agustina.tangapo[at]unsrat.ac.id

(b) Center for the Study of Biotechnology and Conservation of the Wallacea Region, Sam Ratulangi University, Manado, Indonesia.

Abstract

Endophytic bacteria are one of the microbes that powerful to develop their roles as plant growth promoting activities including to produces plant hormone - IAA. The objective of this study was to test in vitro the ability of endophytic bacteria isolated from mangrove plants in North Sulawesi for producing IAA. The in vitro test for analysis of IAA production was determined using Salkowski method measured by spectrophotometer. In this paper, eighteen (18) endophytic bacteria isolated from surface-sterilized leaves of mangrove plants were screened for their ability to produce IAA. These isolates could produce different IAA with different morphological and microscopic characteristics. The results showed that 12 isolates were capable to produce IAA by adding L-Tryptophan with the highest and lowest concentrations were 116.55 ppm (EB17) and 1.25 ppm (EB4), respectively.

Keywords: endophytic bacteria- mangrove- plant growth promoting- plant hormone

Topic: Food Microbiology and Biotechnology